Primer data from Pashley et al. (2006; J Hered 97: 381-388) - Data are presented as tab-delimited text - Markers should be referred to by their Locus_ID - Old_ID reflects nomenclature of Ellis et al. (2006; Mol Ecol: 15: 2345-2355) - Contig_ID refers to the CGP1 Helianthus assembly at http://cgpdb.ucdavis.edu/sitemap.html - The forward primer sequence does not include the M13 tail used for labeling - Expected size is derived from the EST sequences, and will thus underestimate true if the primers flank an intron Locus_ID Old_ID Contig_ID Forward_Primer Reverse_Primer Repeat_Motif Expected_Size Tm_Fwd Tm_Rev HT1092 BL0001 Contig00019 CTGCGTCGGCCTCGATCC GACTTCAACGCCGTATCCAT (aac)4 292 67.7 60 HT1099 BL0002 Contig00185 GGCTTTCGTTTCTCGTTGTC CAGCTCACTCCTAATTGGTTCC (taat)5 302 59.9 60.1 HT1101 BL0003 Contig00346 GCAGTGCTTCCACAGCAAC AACCATTTCAACTCACTAAATAATCTC (atg)4 140 60.6 57.6 HT1106 BL0004 Contig00670 ATCCTCGGCTCAGCTAAACA ATGATGGAGCCACCTATGGA (tcc)4 305 60 60.3 HT1107 BL0005 Contig00902 ACAAGCTCAAACGCCTTCAT CAATTGATGTTCCAAGCAAA (atg)12 253 59.9 57.2 HT1109 BL0006 Contig00918 CATGGGTGATCAATGGAGTG CGGCACATAACAAGTGCTTC (gtga)3 267 59.8 59.3 HT1110 BL0007 Contig00994 CGCAACGGGTCATCTACAG GGACCGAACCCATCTAACAG (ata)4 146 60.3 59.4 HT1111 BL0008 Contig01017 GTCATGAAACCACGGCCTAC TCACCACAAACCTCTCATCTTG (atg)4 436 60.4 60.1 HT1112 BL0009 Contig01020 GTGTCGGGAGTTTGTTGGTC TCATTGCACCCTCTAACACG (tgg)4 402 60.4 59.7 HT1114 BL0010 Contig01258 TCCGAGCTTCCAATCATACC GGAATGGCAATGATGTCTCC (caa)4 264 60 60.3 HT1117 BL0011 Contig01351 TGTTTGCTCACCTTGCTGAC TGACACACAAACACCACTTGC (gcat)3 116 60 60.7 HT1120 BL0012 Contig01381 TGCAGTACTCGGGTGATGAG TGTGATCATACAACACAATGATGA (atta)4 270 59.9 58.8 HT1121 BL0013 Contig01387 CGAGACGGTTAAGAGCTTGC GGTGTACAACCAACTCACACC (gtta)3 355 60.1 57.8 HT1123 BL0014 Contig01406 GGGTTTGTACCAGGCACTTG TTCATAGAAATGAGGACCAAAGG (ggt)4 322 60.4 59.5 HT1127 BL0015 Contig01649 AATTGGAGCGGATGGTATTG AATATCTCTTATTTCAATAGTCCAACG (atg)4 373 59.8 57.6 HT1128 BL0016 Contig01666 GGAGCCGGTTTCTGAATATG GGTGCCTGTGTTTCTTCCTC (tga)4 170 59.5 59.7 HT1129 BL0017 Contig01700 TTGAACAAGTTCGCAAGCTG CAATACTACATCATATAATCGACCAAC (tga)4 174 60.2 57.3 HT1130 BL0018 Contig01715 CATGGTGCATTTGGCTACC TGCTCCTGAAAGAACCCAAG (gca)4 212 59.9 60.4 HT1131 BL0019 Contig01765 GAGTCCTGGCCTGAACAGAG CAAACTGCAATGTACCTTCTTGAC (gaaa)3 311 60 60.1 HT1132 BL0020 Contig01774 AACCATGTTGCTTCCCAATC TTCGGGACCGAGAGTATTTG (cca)4 293 59.8 60.1 HT1134 BL0021 Contig01828 GAGGGTGGCTGTTTCCTATG TTTGATTGCATTGGTGATCC (tggc)4 142 59.5 59.3 HT1135 BL0022 Contig01831 ACTTACCGTTGCATTTGGTG GCTTATCCCTAGAACACGATTACAG (taa)4 116 58.5 59.6 HT1136 BL0023 Contig01905 TTGGGTCAACACAAGCAGTC AAACTGGCATTGGAAAGGTG (tta)4 118 59.7 60 HT1137 BL0024 Contig01921 CTCCCGCACTTCAAGCTAAC CATACACCTTTGCGGTTTCC (gtaa)3 126 60 60.4 HT1138 BL0025 Contig01928 AGTGGTCGGACGGTAAACAC AACACACACCAAATCAAATCAC (tggt)3 208 59.9 57.4 HT1139 BL0026 Contig01946 GGACACCCAATCGATCAAAC CTCCACGCTCTTCTTCGTTC (aag)4 229 60.2 60.1 HT1140 BL0027 Contig01984 AATGTTTCACCGCCTACAGC GCCACCGGCTATATCTCAAC (tgg)5 295 60.1 59.6 HT1141 BL0028 Contig02015 CAGGCTGAAGGATGTTGAAA ACGCGTTATAAGCCGAAACA (ggca)3 363 58.8 60.6 HT1143 BL0029 Contig02031 AAGTCGCACAGGAAGGAGATAG TTGCTTGGAAGCCAATGAG (ttgt)3 101 59.9 59.9 HT1144 BL0030 Contig02070 GGGTAATGCAAAGTACTAAGATGTG GCATCATCCAACAAACTAGAAGG (atgt)3 235 58.3 60 HT1146 BL0031 Contig02095 CCGGAAGATAACGACGAGTG TCCATCGCTTTCCCTAAATC (gac)4 443 60.7 59.1 HT1148 BL0032 Contig02165 GGATATTCGCAGGATGGAAG CTGGCCGTCCTTGTTGAC (atg)4 352 59.5 60.2 HT1150 BL0033 Contig02236 GGGAGTTACACGCCTCCAG CACAACCATACGCCATCAAG (cac)4 278 60.7 60 HT1151 BL0034 Contig02305 GGTCGTCTACTACGGCTTCG TAACCGAACGACCATTCTTC (tgtt)4 166 59.9 57.7 HT1152 BL0035 Contig02307 TGCTCTGATGTTTCGTCCAC TCAAGCCTTTACACAGTTTAGACA (ta)5 331 59.8 58.2 HT1181 BL0036 Contig03472 GAAGGAGGAGTGCGGGTAG TTGCAACTTCGGGAATAAAC (taa)4 200 59.8 57.7 HT1183 BL0037 Contig03626 GGTTAGGGTGAGGGTGGTG AAGCCATAGTAAGTTCCTCTTACAAAC (tgca)3 164 60.2 58.9 HT1187 BL0038 Contig03798 CGCTGCTAAACCAGAAGAGG TTAAACACAGCGCACAAAGC (tttc)3 393 60.2 60.1 HT1191 BL0039 Contig03900 CGAACGGGAGTTGAGGTAGG TGGTGAACAACAAGGTTGGAG (gaat)3 183 61.9 61 HT1192 BL0040 Contig03941 TGCAAGTATGCGATCAGAGAG GAGCAAACGCCTGGTACTTC (gat)4 193 59.2 59.9 HT1196 BL0041 Contig04042 ACATTTGGACGTTTGGAAGC TCCATCGAGATGTTGACACG (ctt)4 192 60 60.7 HT1198 BL0042 Contig04125 GGTTACAACGGTGGAAGTCG TCCGGTTCACCAATTCATTC (ggc)4 373 60.4 60.7 HT1200 BL0043 Contig04142 GAGGCAAAGGAAGTGACAGC GGGATTTAACAGCAATTCATGG (gcct)3 346 60 60.6 HT1201 BL0044 Contig04184 CCGAGGCGGAGAGTAATATG AAGGCTTGCTTTCCTCACAG (tgtt)3 355 59.7 59.6 HT1210 BL0045 Contig04362 AGGATTTGGTGGTGGTTCAG TTAGTAGCAATCAACTCACTACTATGC (tgca)4 119 59.8 57.6 HT1212 BL0046 Contig04398 GAACCAACACAACCAAATCC TGTCGCTTCAACGCATAAAC (aaca)3 334 57.7 59.9 HT1217 BL0047 Contig04479 GACCGAATAGGTTTCAACGA TGCCTCTGCTCTCCTCTTTC (ct)6 314 57.7 59.8 HT1222 BL0048 Contig04644 TTGTGGAGACGGTGAATGAG TCTGCCCGTAGAAACCAAAC (gaa)4 224 59.7 60.1